Antibodies
Tenovin-1
- 貨號 Cat. No.
- S8000
- 規格 Size
- 5~200mg
- 品牌 Brand
- Selleckchem
Description
Tenovin-1
Catalog No.S8000
Tenovin-1 protects against MDM2-mediated p53 degradation, which involves ubiquitination, and acts through inhibition of protein-deacetylating activities of SirT1 and SirT2.
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Distributor Tel: +886-2-2827-1197jimmy@new.abscience.com.tw
Biological Activity
| Description | Tenovin-1 protects against MDM2-mediated p53 degradation, which involves ubiquitination, and acts through inhibition of protein-deacetylating activities of SirT1 and SirT2. | |||||
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| Targets | p53 | |||||
| IC50 | ||||||
| In vitro | Tenovin-1 is a p53 activator and elevates the amount of p53 protein within 2 hr of treatment. Tenovin-1 treatment does not alter p53 mRNA levels. Tenovin-1 (10μM) protects p53 from mdm2-mediated degradation with little effect on p53 synthesis. Tenovin-1 represses cell growth, and induces apoptosis in a panel of tumor cells expressing p53. Tenovin-1 acts through inhibition of the protein-deacetylating activities of SirT1 and SirT2, two important members of the sirtuin family. [1] | |||||
| In vivo | Initial in vivo experiments indicated that tenovin-1 impairs the growth of BL2-derived tumor xenografts. [1] | |||||
| Features | ||||||
Protocol(Only for Reference)
Cell Assay: [1]
| Cell lines | HCT116, HCT116 p53−/−, EW36, BL2, NTera2D, NTera2D-DNp53, ARN8, NHDF, SKNH-pCMV and SKNSH-DNp53. |
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| Concentrations | 10 μM |
| Incubation Time | 4 days |
| Method | Cell viability isdetermined by trypan blue exclusion, Giemsa staining, or MTT assays. |
Animal Study: [1]
| Animal Models | Female SCID mice injected subcutaneously with ARN8 cells suspended in matrigel. |
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| Dosages | 92.5 mg/kg |
| Administration | i.p. |
| Solubility | 15% Captisol, 15 mg/mL |
References
Chemical Information
| Molecular Weight (MW) | 369.48 |
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| Formula | C20H23N3O2S |
| CAS No. | 380315-80-0 |
| Storage | 3 years -20℃Powder |
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| 6 months-80℃in DMSO | |
| Syonnyms | N/A |
| Solubility (25°C) * | In vitro | DMSO | 74 mg/mL (200 mM) |
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| Water | <1 mg/mL (<1 mM) | ||
| Ethanol | <1 mg/mL (<1 mM) | ||
| In vivo | 15% Captisol, | 15 mg/mL | |
| * <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. | |||
| Chemical Name | Benzamide, N-[[[4-(acetylamino)phenyl]amino]thioxomethyl]-4-(1,1-dimethylethyl)- |
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Preparing Stock Solutions
| Stock Solution (1ml DMSO) | 1mM | 10mM | 20mM | 30mM |
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| Mass(mg) | 0.36948 | 3.6948 | 7.3896 | 11.0844 |
