TCR alpha Antibody

Antibody Target: TCRa

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 138 of human TCRα

Accession#: 18195

Gene ID: 171558

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IP/IHC

Application & Usage: Western blotting (0.5-4 µg/ml). Based on researcher’s feedback, it can also be used in immunoprecipitation (10-20 µg/ml); and immunohistochemistry (15-25 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes TCR α in human sample, and to a lesser extent to mouse and rat samples. Jurkat cell lysate can be used as a positive control.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: TCR (T cell antigen receptor) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and &delta: chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while roughly 2-5% express the γ/&delta: heterodimer. CD3 chains and the CD4 or CD8 coreceptors are also required for efficient signal transduction through the TCR.