TaiCatoxin
- 貨號 Cat. No.
- T-800
- 規格 Size
- 0.1 mg 5x0.1mg
Description
TaiCatoxin
Specific and Reversible Blocker of High Voltage Activated Ca2+ Channels
| Product#: | T-800 |
| Sizes: |
| 0.1 mg |
| 5 x 0.1 mg |
Taicatoxin was originally shown to be a specific and reversible blocker of high voltage activated Ca2+ channels in guinea pig and rat ventricular myocytes (probably Cav1.2).1 The IC50 is voltage dependent decreasing from 500 nM at holding potential =-80 mV to 10 nM at holding potential = -30mV, indicating preference of block of the inactivated state of the channels.1 However, another report3 shows that 50 nM Taicatoxin completely blocked SKCa apamin-sensitive channel.in chromaffin cells. Electrophysiological recordings were used to monitor Ca2+ currents1 or action potential parameters4 from isolated myocytes and from chromaffin cells3. In addition, TaiCatoxin was used to displace 125I-Apamin binding in rat brain synaptosomes.3
| M.W.: | 52000 |
| Purity: | >97% |
| Bioassay: |
Taicatoxin was tested for its ability to block Cav1.2 channels expressed in Xenopus oocytes. |
 |
Left: Example traces before (blue) and during (pink) application of 2 mM toxin. Right: Time course of the of current amplitude inhibition. Toxin was perfused at 2 mM at periods indicated by the bars. |
| References: |
1. | Possani, L.D. et al. (1992) Toxicon 30, 1343. |
2. | Lowry, O.H. et al. (1951) J.Biol.Chem. 193, 265. |
3. | Doorty, K.B. et al. (1997) J.Biol.Chem. 272, 19925. |
4. | Athias, F.E. et al. (1996) Mol. Cell Biochem. 160, 61. |
TaiCatoxin is isolated from Oxyuranus s.scutellatus snake venom, by modification of the procedure of Possani1 and purified to homogeneity.