TACE Antibody

Antibody Target: TACE/ADAM17

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 810 of human TACE

Accession#: P78536

Gene ID: 6868

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-TACE polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB

Application & Usage: Western blotting (1-2 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects 80-130 kDa bands, which may represent mature protein, precursor and glycosylated TACE. HeLa and Jurkat cell lysate can be used as positive control.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Tumor necrosis factor-α (TNF-α) is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bond metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGF-α, L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues.