Syndecan-4 Antibody

Antibody Target: Syndecan-4

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 184 of human Syndecan-4

Accession#: P31431

Gene ID: 6385

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) Protein A purified rabbit anti-Syndecan-4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB/IP/IHC

Application & Usage: Western blotting (0.5-4 µg/ml), immunoprecipitation (5-10 µg/ml) and immunohistochemistry (Frozen and paraffin sections; 15-25 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.