Surfactant Protein A Antibody (LS-B2543)

貨號 Cat. No.: LS-B2543
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2543
Antibody Validation:	This antibody replaces catalog number LS-C17957. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Lung Surfactant Protein A (SFTPA1)
Synonyms:	SFTPA1, pulmonary surfactant-associated protein a
Host:	SFTPA1 antibody was produced in Mouse
Antigen Species:	SFTPA1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Purified protein
Immunogen:	SFTPA1 antibody was raised against native SP-A purified from bronchioalveolar lavage from proteinosis patients.
Antibody Specificity:	Recognizes human pulmonary SP-A. In Western Blot after SDS-PAGE it reacts with reduced forms of SP-A and weaker with non-reduced forms. In immunohistochemical staining from paraffin embedded tissue samples, this antibody reacts specifically with human SP-A. Immunoreactivity is seen with alveolar type II cells, nonciliated bronchial cells and a subset of alveolar macrophages. No reactivity in samples from pancreas, intestines, thymus, prostate gland or the peritoneal cavity lining cells. Reacts strongly with SP-A when tested in ELISA with SP-A directly coated onto the microtiter well.
Purification:	Protein A/G Column
Presentation:	PBS, pH 7.4, 15mM sodium azide
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2543 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2543 was determined to be 10 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1:500), ELISA (1:8000) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml