SUMO3-agarose
- 貨號 Cat. No.
- UL-765
- 規格 Size
- 0.5 ml
- 品牌 Brand
- Boston Biochem
Description
SUMO3-agarose
human recombinant
Product Information
0.5 ml
SUMO3 coupled to agarose at 5 mg/ml resin (0.45 mM). Resin supplied in 50 mM Hepes pH 7.5, 250 mM NaCl.
> 95% by SDS-PAGE
Use & Storage
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.
Catalog #: UL-765
SUMO3-agarose
Product Source:
human recombinant
SUMO3 covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for isolation and capture of SUMO3 interacting proteins such as the SUMO activating E1 enzyme, the SUMO carrier enzyme Ubch9, SUMO E3 ligases, SENPs and other proteins/enzymes that have an affinity for SUMO proteins.
Product Information
Quantity:
0.5 ml
Stock:
SUMO3 coupled to agarose at 5 mg/ml resin (0.45 mM). Resin supplied in 50 mM Hepes pH 7.5, 250 mM NaCl.
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and
elution of material is dependent on individual experimental conditions.
Storage:
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.