SUMO2, human recombinant

Alternate Names / Synonyms: SUMO-2, Ubiquitin-like protein SMT3B, SMT3 homolog 2, Sentrin-2, HSMT3, SUMO-3, Sentrin2, SUMO2, SMT3H2, MGC117191.

Gene Symbol: SUMO2

Accession #: P61956

Gene ID: 6613

Gene Source: Human

Recombinant Host: E. coli

Appearance: Liquid

Physical Form: In 50 mM HEPES, pH 8.0, plus 150 mM NaCl, 1 mM DTT.

Molecular Weight: 11.6 kDa

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: <0.1 ng/μg

Biological Activity: Typical in vitro concentrations for conjμgate formation is 10-50 µM depending on conditions.

Reconstitution: N/A

Storage Temp.: -80°C

Shipping: Blue ice

Background Information: SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. Still, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of Cellular processes, for example nuclear transport, transcriptional regulation, apoptosis, and protein stability. The active recombinant SUMO-2 is derived from the precursor pro-SUMO-2 (Accession # NM_006937). Human SUMO-2 shares 44% and 86% identity with SUMO-1 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.

Handling: Centrifuge the vial prior to opening.