SUMO Protein Set
- 貨號 Cat. No.
- K-700
- 規格 Size
- 1 kit
- 品牌 Brand
- Boston Biochem
Description
SUMO Protein Set
human recombinant
Product Information
1 kit
50mM HEPES pH 8.0, 150mM NaCl, 1 mM DTT.
> 95 % by SDS-PAGE
Use & Storage
Typical concentration to support conjugation reaction in vitro is 10 μM-50 μM depending on conditions.
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: K-700
SUMO Protein Set
Product Source:
human recombinant
The ubiquitin-like SUMO-1, SUMO-2 and SUMO-3 proteins are conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. Human SUMO-1 shares 46% and 47% identity with SUMO-2 and SUMO-3 respectively. SUMO-1 is usually conjugated to proteins as a monomer. SUMO-2 shares 86% identity with SUMO-3 and these isoforms are known to form SUMO chains. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4, E2-25K). Unlike ubiquitination, SUMOylation does not appear to target proteins for degradation by the proteasome. SUMO modification has been implicated in diverse functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein stability.
Product Information
Quantity:
1 kit
Stock:
50mM HEPES pH 8.0, 150mM NaCl, 1 mM DTT.
Purity:
> 95 % by SDS-PAGE
Supplied:
| Protein | MW | Concentration | Quantity |
| SUMO-1 | 11.1 kDa | 2.8 mg/ml (250 μM) | 100 μg |
| SUMO-2 | 10.5 kDa | 2.63 mg/ml (250 μM) | 100 μg |
| SUMO-3 | 10.6 kDa | 2.65 mg/ml (250 μM) | 100 μg |
Use & Storage
Use:
Typical concentration to support conjugation reaction in vitro is 10 μM-50 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.