SUMO Conjugation Peptide Substrate, Biotinylated
- 貨號 Cat. No.
- SP-300
- 規格 Size
- 20 μg
- 品牌 Brand
- Boston Biochem
Description
SUMO Conjugation Peptide Substrate, Biotinylated
Synthetic
Product Information
20 μg
1.53 kDa, (11 residues in length)
Soluble in aqueous buffer up to 5 mg/ml.
This is a lyophilized powder
Use & Storage
Add stock to in vitro or in vivo assays at desired concentration. Recommended concentration range is 50-100 μM depending on conditions.
Store stock solution at -20°C. Avoid freeze/thaw cycles.
≥ 96.2 % by HPLC.
Catalog #: SP-300
SUMO Conjugation Peptide Substrate, Biotinylated
Product Source:
Synthetic
This biotinylated peptide contains a sumoylation consensus sequence and can be covalently conjugated to SUMO-1, SUMO-2 and SUMO-3 proteins in the presence of the E1 activating (SAE1/SAE2) and UbcH9 conjugating enzymes. The biotin group allows for convenient and sensitive detection with avidin-linked reagents.
SUMO modification of proteins occurs on lysine residues generally found within a short consensus sequence containing the ψΚXE motif. This motif consists of ψ which represents a large hydrophobic amino acid (isoleucine, leucine or valine), K is the lysine that becomes modified, X is any residue and E is glutamic acid. The glutamic acid is the most highly conserved position other than the lysine.
Product Information
Quantity:
20 μg
MW:
1.53 kDa, (11 residues in length)
Solubility:
Soluble in aqueous buffer up to 5 mg/ml.
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Add stock to in vitro or in vivo assays at desired concentration. Recommended concentration range is 50-100 μM depending on conditions.
Storage:
Store stock solution at -20°C. Avoid freeze/thaw cycles.
Purity:
≥ 96.2 % by HPLC.