SUMO Activating Enzyme (E1)
- 貨號 Cat. No.
- E-315
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
SUMO Activating Enzyme (E1)
human recombinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM Hepes pH 8, 200 mM NaCl, 1 mM DTT, 10% glycerol. Actual concentration will vary with specific lot number.
110 kDa
> 90% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Catalog #: E-315
SUMO Activating Enzyme (E1)
Product Source:
human recombinant
Also known as: SUMO Activating Enzyme (SAE1/SAE2), or UBA2
Conjugation of the ubiquitin-like modifier SUMO (Sentrin) requires the activities of the heterodimeric E1 (SAE1/SAE2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high- energy thiolester bond with the cysteine residue of SAE2 and the concomitant release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM Hepes pH 8, 200 mM NaCl, 1 mM DTT, 10% glycerol. Actual concentration will vary with specific lot number.
MW:
110 kDa
Purity:
> 90% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.