SUMO-3 Conjugation Kit
- 貨號 Cat. No.
- K-720
- 規格 Size
- 1 kit
- 品牌 Brand
- Boston Biochem
Description
SUMO-3 Conjugation Kit
human recombinant
Product Information
1 kit
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Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: K-720
SUMO-3 Conjugation Kit
Product Source:
human recombinant
This kit is designed for the conjugation of the ubiquitin-like modifier SUMO-3 to protein substrates in vitro, which requires the activities of the heterodimeric human E1 activating enzyme (SAE1/SAE2) and the UbcH9 E2 enzyme.
NOTE: Kit contains reagents sufficient for 5-10 x 20 μl reactions.
Product Information
Quantity:
1 kit
Western Blot Data:
Western Blot Data:
Supplied:
| Protein | Concentration | Quantity |
| 10X E1 Enzyme | X mg/ml (X μM) | 20 μL |
| 10X SUMO-3 | X mg/ml (X μM) | 20 μL |
| 10X UbcH9 | X mg/ml (X μM) | 20 μL |
| SUMO-1 K17R | X mg/ml (X μM) | 20 μL |
| 10X Mg-ATP Solution | X mM | 20 μL |
| 10X Reaction Buffer | X mM Hepes pH 8 | 20 μL |
| X mM NaCl | ||
| X mM DTT |
Background:
The ubiquitin-like SUMO-3 is conjugated to a variety of proteins in the presence of UbcH9 and the SUMO E1 activating enzyme (SAE1/SAE2 in human, or Aos1/Uba2p in yeast). This isoform is known to form poly-SUMO chains on substrates via isopeptide bond formation between the Cterminal glycine of one SUMO-3 to the lysine-ï€ ε-amino group of K11 on the preceding SUMO-3. The heterodimeric SAE1/SAE2 complex (38 and 70 kDa respectively) uses ATP to adenylate the Cterminal glycine residue of SUMO-3, forming a high-energy thiolester bond with the SAE2 subunit. The second step is the trans-esterification reaction whereby the activated SUMO-3 is transferred to Cys93 of UbcH9. UbcH9 is a member of the E2 family and is homologous to ubiquitin-conjugating enzymes, but is specific for the conjugation of SUMO to a variety of target proteins. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Sumoylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates(IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation, apoptosis and protein function and stability.
Use & Storage
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.