SUMO-1 K7R mutant
- 貨號 Cat. No.
- ULM-710
- 規格 Size
- 250 μg
- 品牌 Brand
- Boston Biochem
Description
SUMO-1 K7R mutant
human recombinant
Product Information
250 μg
X mg/ml (XμM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
11.1 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.
Catalog #: ULM-710
SUMO-1 K7R mutant
Product Source:
human recombinant
Human SUMO-1 does not contain the exact ψΚXE consensus sequence found in SUMO-2 and SUMO-3. Within this motif ψ represents a large hydrophobic amino acid (I, L, or V), K is the lysine that becomes modified, X is any residue and E is glutamic acid. Many known SUMO-1 conjugation sites occur within this consensus sequence, but SUMOylation also occurs on lysine residues located within non-consensus regions. SUMO-1 has been shown to form chains in vitro and in vivo but often the linkage is uncharacterized, and the function of SUMO chains has not yet been fully elucidated. SUMO-1 multimerization in vitro has been shown to occur predominantly via lysines K7, K16 and K17. Mutation of lysine 7 to arginine is useful to investigate mono- SUMOylation requirements or to reduce poly-SUMO chain formation.
Product Information
Quantity:
250 μg
Stock:
X mg/ml (XμM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW:
11.1 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Storage:
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.