SUMO-1 K7R K16R mutant
- 貨號 Cat. No.
- ULM-716
- 規格 Size
- 250 μg
- 品牌 Brand
- Boston Biochem
Description
SUMO-1 K7R K16R mutant
human recombinant
Product Information
250 μg
X mg/ml (XμM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
11.1 kDa
> 95% by SDS-PAGE
Use & Storage
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.
Catalog #: ULM-716
SUMO-1 K7R K16R mutant
Product Source:
human recombinant
Human SUMO-1 does not contain the exact ψΚXE consensus sequence found in SUMO-2 and SUMO-3. Within this motif ψ represents a large hydrophobic amino acid (I, L, or V), K is the lysine that becomes modified, X is any residue and E is glutamic acid. Many known SUMO-1 conjugation sites occur within this consensus sequence, but SUMOylation also occurs on lysine residues located within non-consensus regions. SUMO-1 has been shown to form chains in vitro and in vivo but often the linkage is uncharacterized, and the function of SUMO chains has not yet been fully elucidated. SUMO-1 multimerization in vitro has been shown to occur predominantly via lysines K7, K16 and K17. Mutation of lysine 17 to arginine is useful to investigate monosumoylation requirements or can be used to reduce poly-SUMO chain formation.
Product Information
Quantity:
250 μg
Stock:
X mg/ml (XμM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW:
11.1 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Storage:
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.