SUMO-1 AMC

貨號 Cat. No.
UL-551
規格 Size
50 μg
品牌 Brand
Boston Biochem

Description

SUMO-1 AMC

Catalog #: UL-551




SUMO-1 AMC






Product Source: 

human recombinant



Fluorogenic substrate for SUMO-1 hydrolases based on the carboxy-terminus derivatization of SUMO-1 with 7-amido-4-methylcoumarin (AMC). SUMO-1 AMC is useful for studying SUMO-1 hydrolases when detection sensitivity or continuous monitoring of activity is essential.


Product Information



Quantity: 

50 μg






Stock: 

X mg/ml (X μM) in 100 % DMSO Concentration varies with lot number.






MW: 

11.6 kDa






Purity: 

> 95% by HPLC




 


Use & Storage



Use: 

Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.






Storage: 

Store at -80°C. Avoid multiple freeze/thaw cycles.



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