SUMO-1 Agarose
- 貨號 Cat. No.
- UL-740
- 規格 Size
- 0.5 ml
- 品牌 Brand
- Boston Biochem
Description
SUMO-1 Agarose
human recombinant
Product Information
0.5 ml
[SUMO-1] = 5 mg/ml resin (431 μM). SUMO-1 agarose is supplied in a 20% ethanol solution.
> 95% by SDS-PAGE
Use & Storage
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and
elution of material is dependent on individual experimental conditions and
goals.
SUMO1-agarose can be re-used for at least 10-15 applications if properly maintained. After use, clean resin by washing with 10 ml 50 mM Tris-Cl pH9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing either 1 mM NaN3 or 10-20 % ethanol. Resin should be stored at 4°C. DO NOT FREEZE
Catalog #: UL-740
SUMO-1 Agarose
Product Source:
human recombinant
SUMO-1 is covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Useful for affinity binding of SUMO-1 activating enzyme, SUMO conjugating E2 enzyme Ubch9, SUMO-1 ligases, SUMO C-terminal hydrolases, and other proteins/enzymes that have an affinity for SUMO-1. The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of Ubch9 and the SAE1/SAE2 activating enzyme. SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation.
Product Information
Quantity:
0.5 ml
Stock:
[SUMO-1] = 5 mg/ml resin (431 μM). SUMO-1 agarose is supplied in a 20% ethanol solution.
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and
elution of material is dependent on individual experimental conditions and
goals.
Storage:
SUMO1-agarose can be re-used for at least 10-15 applications if properly maintained. After use, clean resin by washing with 10 ml 50 mM Tris-Cl pH9.0, 1 M KCl. Remove cleaning solution by equilibrating resin in 25 mM HEPES (or similar buffer) pH 7.5, containing either 1 mM NaN3 or 10-20 % ethanol. Resin should be stored at 4°C. DO NOT FREEZE