Sucrose Colorimetric/Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K626-100
- 規格 Size
- K626-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 monitoring level during fermentation and feeding in protein expression processes 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
No interference from Lactose & Maltose
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The method can detect 0.0002-10 mM sucrose concentrations.
Detection Method:
Features & Benefits:
• Simple procedure; takes ~1 hour
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Sucrose
Description:
Sucrose (C₁₂H₂₂O₁₁; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose. BioVision’s Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.