Suc-Leu-Tyr-AMC (Suc-LY-AMC)
- 貨號 Cat. No.
- S-260
- 規格 Size
- 5 mg
- 品牌 Brand
- Boston Biochem
Description
Suc-Leu-Tyr-AMC (Suc-LY-AMC)
Synthetic
Product Information
5 mg
Soluble in DMSO and MeOH (recommend DMSO stock solution). Pellet dry compound prior to DMSO addition.
Use & Storage
Store DMSO stock at -20°C. Avoid multiple freeze/thaw cycles.
> 95% by TLC, HPLC. Structure confirmed by NMR.
Catalog #: S-260
Suc-Leu-Tyr-AMC (Suc-LY-AMC)
Product Source:
Synthetic
Fluorogenic substrate for measuring the chymotrypsin-like peptidase activity of the 20S proteasome. The 20S complex is composed of 28 subunits, arranged in anï€ α7β7β7α7 stoichiometry. Each of the two internal β-type rings harbors three different proteolytically active sites, provided by the amino-terminal residues of three constitutive subunits: β1 (post-glutamyl peptide hydrolase site), β2 (trypsin-like site) and β5 (chymotrypsin-like site). Also a peptide substrate for the Ti protease
from E.coli.
Product Information
Quantity:
5 mg
Structure (deprecated):
Formula: C29H31N3O7 Formula Weight: 533.59
Solubility:
Soluble in DMSO and MeOH (recommend DMSO stock solution). Pellet dry compound prior to DMSO addition.
Use & Storage
Use:
Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)
Storage:
Store DMSO stock at -20°C. Avoid multiple freeze/thaw cycles.
Purity:
> 95% by TLC, HPLC. Structure confirmed by NMR.