Streptococcus pyogenes M1 CRISPR-Cas9 Protein (His Tag)_40572-A08B

分子 : CRISPR-Cas9

保存條件 : Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

蛋白構建: A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.

纯度: > 90 % as determined by SDS-PAGE.

内毒素 : < 1.0 EU per μg protein as determined by the LAL method.

預測N端 : Met

缓冲液 : Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.

NP號 : WP_010922251.1

氨基酸序列 : Met1-Asp1368

標籤 : C-His