Streptavidin-Phycoerythrin, SAv-PE

Description

Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.

Product Details

Reactivity

Human, Mouse, Rat, All Species

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

Streptavidin is conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)

Storage & Handling

The streptavidin-PE solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE is 360 kD and Streptavidin alone is 52 kD.