STEAP1 Antibody (LS-B2828)

貨號 Cat. No.: LS-B2828
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2828
Antibody Validation:	This antibody replaces catalog number LS-C56889. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Six Transmembrane Epithelial Antigen Of The Prostate (STEAP) (STEAP1)
Synonyms:	STEAP1, MGC19484, PRSS24, six transmembrane epithelial antigen of the prostate, steap
Host:	STEAP1 antibody was produced in Rabbit
Antigen Species:	STEAP1 antibody was raised against Human
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide
Immunogen:	STEAP1 antibody was raised against an 18 amino acid peptide located near the c-terminal region of human STEAP1
Antibody Specificity:	Recognizes STEAP1 (six transmembrane epithelial antigen of the prostate), a 39kD protein which is a member of a family of cell surface serpentine transmembrane antigens of the prostate. STEAP1 is predominantly expressed in human prostate and is up-regulated in several cancer lines including prostate, ovarian, cervical, breast, testicular, bladder, colon and Ewing sarcoma. It is significantly expressed at cell-cell junctions and has been shown to be a target of human CD8 T cells. This indicates that it could be used as a potential target for cancer immunotherapy. This antibody does not cross-react with other STEAP-proteins
Antigen Modification:	C-Terminus
Predicted Species Reactivity:	Mouse, Rat
Purification:	Affinity Chromatography
Presentation:	PBS, 0.02% sodium azide.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2828 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2828 was determined to be 5 ug/ml. Western Blot: LS-B2828 detects a band of approximately 40kD in human spleen cell lysates.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 - 2 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml