Staurosporine
- 貨號 Cat. No.
- S-350
- 規格 Size
- 10 μg 50 μg 5x50 μg 0.47mg 5x0.1mg 0.1mg
Description
Staurosporine
| Product#: | S-350 |
| Sizes: |
| 10 x 10 mg |
| 50 mg |
| 5 x 50 mg |
| 0.47 mg |
| M.W.: | 466 daltons. |
| CAS No.: | [62996-74-1] |
| Structure: |
| . |
 |
| Purity: | Greater than 99% as evaluated by HPLC. |
| Bioassay: |
| The biological activity was tested to confirm the ability of Staurosporine to induce apoptosis and inhibit phosphorylation. |
 |
Jurkat cells were grown to 70% confluency. Then 2 mM Staurosporine or vehicle were added for 3 or 6 hours. At the end of the incubation period, the cells extracts were detected for Caspase 3 and cleaved Caspase 3 with specific antibodies.
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| Staurosporine inhibits the serine phosphorylation of Myristoylated alanin-rich protein kinase C substrate (MARCKS). C6 glioma cells were grown to 70% confluency and then serum starved for 18 h. Then the cells were preincubated for 30 min in different concentrations of staurosporine and stimulated with TPA (2 mM) for 30 min. Cells extracts were prepared for Western blotting and the proteins were probed with an antibody against Phospho-(Ser)- Myristoylated alanin-rich protein kinase C substrate (P-MARCKS). |
| References: |
1. | Ruegg U. T. and Burgess, G. M. (1989) Tips 10, 218. |
2. | Tamakori, T. et al. (1986) B.B.R.C. 135, 397. |
3. | Matsumoto, H. et al. (1989) B.B.R.C. 158, 105. |
4. | Couldewell, W. T. et al. (1994) FEBS Lett. 345, 43. |
5. | Bruno, S. et al. (1992) Cancer Res. 52, 470. |
6. | Vitale, M. L. et al. (1992) Neuroscience 51, 463. |
7. | Hoffman, R. and Newland, E. S. (1991) Cancer Chemother. Pharmacol. 28, 102. |
8. | Begemann, M. et al. (1998) Anticancer Res. 18, 3139. |
9. | Oh, Y. J. et al. (1997) Brain Res. Mol. Brain Res. 51, 133. |
10. | Hou, S. T. et al. (2000) J. Neurochem. 75, 91. |
11. | Rasouly, D. et al. (1992) Mol. Pharmacol. 42, 35. |
12. | Ryoji Y. et al. (1997) J. Biol. Chem. 272, 18261. |
13. | Kruman, I. et al. (1998) J. Neurosci. Res. 51, 293. |
14. | Jacobsen, M. D. et al. (1996) J. Cell Biol. 133, 1041. |
15. | Posmantur, R. et al. (1997) J. Neurochem. 68, 2328. |
16. | Chakravarthy, B. R. et al. (1999) J. Neurochem. 72, 933 |
Staurosporine is a member of the K252a family of fungal alkaloids produce by Streptomyces staurospores.1-2 It is one of the most potent, cell permeable inhibitors of protein kinases, and it is often used to study the involvement of protein kinases in signal transduction pathways (IC50 0.7-20 nM).3-10
In pheochromocytoma PC12 cells, neuroblastoma and brain primary culture Staurosporine is a functional neurotropins agonist promoting neurite outgrowth (EC50 = 50 nM).11-12 At concentrations higher then 0.5 mM Staurosporine generates apoptosis in several different cell types or induces G1 and G2 phase arrest in normal cells.13-16