Stat6 Polyclonal Ab

HOST: Rabbit

IMMUNOGEN: Synthetic peptide surrounding amino acid 623 of human Stat6

SPECIES REACTIVITY: human, mouse, rat

FORMULATION: 100 ug (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.

STORAGE CONDITIONS: Store at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.

BACKGROUND: Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3ß appears to be activated by both while Stat3a is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

SPECIFICITY: The antibody recognizes the total level of endogenous Stat6 in samples of human, mouse, and rat origins. Reactivity to other species has not been tested.

APPLICATION: The antibody can be used in Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. USAGE: For Research Use Only. Not to be used in humans.