Proteins and Peptides
STAT5 Antibody (LS-B2431)
- 貨號 Cat. No.
- LS-B2431
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2431 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C60124. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | STAT5A/STAT5B |
| Host: | STAT5A/STAT5B antibody was produced in Rabbit |
| Antigen Species: | STAT5A/STAT5B antibody was raised against Mouse |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide |
| Immunogen: | STAT5A/STAT5B antibody was raised against synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein |
| Antibody Specificity: | A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined. |
| Antigen Modification: | pTyr694 |
| Predicted Species Reactivity: | Human, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2431 was determined to be 20-40 ug/ml. Western Blot: Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein in the appropriate cell lysate or extract. This phosphospecific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (20 - 40 µg/ml), Western blot (WB) (1:500 - 1:2000), ELISA (1:25000) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
同品牌其他產品
