Proteins and Peptides
STAT3 Antibody (LS-B2175)
- 貨號 Cat. No.
- LS-B2175
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2175 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C10392. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Signal Transducer and Activator Of Transcription 3 (STAT3) |
| Synonyms: | STAT3, APRF, signal transducer and activator of transcription 3, stat3 |
| Host: | STAT3 antibody was produced in Mouse |
| Antigen Species: | STAT3 antibody was raised against Mouse |
| Clonality: | Monoclonal |
| Isotype: | IgG2a |
| Antigen Type: | Synthetic peptide |
| Immunogen: | STAT3 antibody was raised against synthetic phospho-peptide corresponding to residues surrounding Ser727 of mouse Stat3. KLH-conjugated. |
| Antibody Specificity: | Recognizes Stat3 when phosphorylated at Serine 727. It does not appreciably cross-react with the corresponding phosphorylated serines of other Stat proteins. This antibody does not react with non-phosphorylated Stat3 or with Stat3 phosphorylated at other sites. Species cross-reactivity: Human and mouse. |
| Antigen Modification: | pSer727 |
| Predicted Species Reactivity: | Human |
| Purification: | Purified |
| Presentation: | 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2175 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2175 was determined to be 1:100. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4uC with gentle shaking, overnight. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:100), Immunocytochemistry (ICC), Western blot (WB) (1:1000), Immunoprecipitation (IP) (1:50) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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