STAT1alpha Polyclonal Ab

HOST: Rabbit

IMMUNOGEN: Synthetic peptide surrounding amino acid 715 of human STAT1a

FORMULATION: 100 ug (0.2 mg/ml) immunoaffinity purified, rabbit anti-STAT1a polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

SPECIES REACTIVITY: human, mouse, rat

STORAGE CONDITIONS: Store at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles.

BACKGROUND: STATs (signal transducers and activators of transcription) are a family of cytoplamic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signalling peptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6). Stat1, Stat3, Stat5a and Stat5b have wide activation profiles. Stat1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-r, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF-1), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Stat1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1߬ which are coded by the same gene with splicing variant.

SPECIFICITY: The immunoaffinity purified antibody detects the 91 kDa STAT1a. No cross reactivity with the 84 kDa STAT1ß®

APPLICATION AND USAGE: The antibody can be used for Western blot analysis (1 ug/ml) and immunoprecipitation (4-8 ug/ml). However, the optimal conditions should be determined individually.

NOTE: For research purpose only! Not to be used in human.