Stat1 Antibody

Antibody Target: STAT1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide surrounding amino acid 672 of rat Stat1

Accession#: AAF20200

Gene ID: 25124

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes both alpha (91 kDa) and beta (84 kDa) subunits of STAT1 in samples from human, mouse, and rat origins.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: STATs (signal transducers and activators of transcription) are a family of cytoplamic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signalling peptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6). Stat1, Stat3, Stat5a and Stat5b have wide activation profiles. Stat1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-r, and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF-1), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Stat1 has two forms, the 91 kDa STAT1α and the 84 kDa STAT1β, which are coded by the same gene with splicing variant.