Sphingosine Kinase 1 (SPK1) Antibody

Antibody Target: Sphingosine Kinase 1 (SK1/SPK1)

Alternative Name: SPK1, SPK-1, SPK 1, sphk, SK1, SK 1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 180 of mouse Sphingosine Kinase 1

Accession#: CAM17115

Gene ID: 20698

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse, rat

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 42 kDa bands from samples of human, mouse and rat origins. Reactivity to other species has not been tested.

Storage Temp.: -20 °C

Shipping: gel pack

Shelf Life: 12 months

Background Descriptions: Sphingosine Kinase (SphK) is a conserved lipid kinase that catalyzes the phosphorylation of sphingolipid sphingosine to sphingosine-1-phosphate (SIP). SIP receptors coupled to cell surface G protein to regulate cell growth, survival, motility and inflammatory responses. There are two types of SphK, SphK1 and SphK2. SphK1 is found in the cytosol and migrates to the membrane when activated. SphK1 has been associated with cell growth, prevention of apoptosis and cellular transformation. SphK2 is found mainly in the nucleus and in contrast to SphK1, it enhances the rate of apoptosis.

Handling: The antibody solution should be gently mixed before use.