Sphingomyelin Quantification Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K600-100
- 規格 Size
- K600-100
- Sample Type
- • Serum 、 plasma • Tissue samples such as brain etc. • Adhesion or suspension cells
Description
Alternate Name :
Tag Line :
The most sensitive kit on the market
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (OD 570 nm)
• Applications- - Sphingomyelin quantification in various biological samples
- Analysis of cell signaling pathway
Detection Method: Absorbance (OD 570 nm)
Features & Benefits:
• Simple, rapid & convenient
• The assay can detect sphingomylein less than 1 µM in a variety of samples
Description:
Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM.