Proteins and Peptides
SNAP25 Antibody (LS-B2464)
- 貨號 Cat. No.
- LS-B2464
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2464 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C10247. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Synaptosomal Associated Protein 25 (SNAP25) |
| Synonyms: | SNAP25, BA416N4.2, DJ1068F16.2, FLJ23079, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, synaptosomal associated protein 25 |
| Family: | SNAP-25 |
| Subfamily: | Not assigned-Other |
| Host: | SNAP25 antibody was produced in Mouse |
| Antigen Species: | SNAP25 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Protein extract |
| Immunogen: | SNAP25 antibody was raised against crude human synaptic immunoprecipitate. |
| Antibody Specificity: | Appears to recognize the human pre-synaptic protein, SNAP-25; an antigen of 26-27kD molecular weight. It will recognize SNAP-25 fusion protein from COS cells, but not with the fusion protein from bacterial systems. Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (1., 3., 8.). Species cross-reactivity: rat, hamster and porcine. |
| Predicted Species Reactivity: | Hamster, Porcine, Rat |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.2, 0.09% sodium azide |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2464 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2464 was determined to be 5 ug/ml. Western Blot: 1:250-1:2500. Positive control: Brain. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1:250 - 1:2500), ELISA (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
| Concentration: | 1 mg/ml |
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