Proteins and Peptides
SMAD7 Antibody (LS-B120)
- 貨號 Cat. No.
- LS-B120
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B120 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C807. It has been validated for use in the following assays: IHC. |
| Antibody Target: | SMAD, Mothers Against DPP Homolog 7 (SMAD7) |
| Synonyms: | SMAD7, madh7, smad7 |
| Host: | SMAD7 antibody was produced in Rabbit |
| Antigen Species: | SMAD7 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | SMAD7 antibody was raised against synthetic peptide |
| Antibody Specificity: | Two synthetic peptides corresponding to aa 12-18 (RLWRSRAPGGEDEEEGAG) and amino acids 36-50 (ELRGEGATDSRAHGA) of human SMAD7; GenBank Accession no. AAB81354.1. This sequence is 100% or highly conserved between human and multiple species. |
| Antigen Modification: | aa36-50 |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Protein G Column |
| Presentation: | PBS, 0.05% BSA, 0.05% sodium azide. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B120 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B120 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1:500 - 1:1000) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |
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