SMAD6 Antibody (LS-B2817)

貨號 Cat. No.: LS-B2817
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2817
Antibody Validation:	This antibody replaces catalog number LS-C10230. It has been validated for use in the following assays: IHC-P.
Antibody Target:	SMAD6
Synonyms:	SMAD6 Antibody, HsT17432 Antibody, MADH6 Antibody, MADH7 Antibody, smad6 Antibody
Host:	SMAD6 antibody was produced in Rabbit
Antigen Species:	SMAD6 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Recombinant protein
Immunogen:	SMAD6 antibody was raised against an approximately 200 amino acid recombinant fragment derived from the amino terminal portion of the human Smad6 protein.
Antibody Specificity:	Specific for Smad6. Cross-reactivity with other Smad family members has not been observed. Species cross-reactivity: Confirmed using human Smad6-transfected COS cells. Endogenous Smad6 is also detected by Western Blot.
Antigen Modification:	N-Terminus
Predicted Species Reactivity:	Mouse
Purification:	Purified
Presentation:	PBS, pH 7.4, 0.1% sodium azide
Recommended Storage:	Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2817 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2817 was determined to be 5-10 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 - 10 µg/ml), Western blot (WB) (3 - 5 µg/ml), Immunoprecipitation (IP), Immunofluorescence (IF) (10 - 15 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg