SMAD6 Antibody (LS-B2065)

貨號 Cat. No.: LS-B2065
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2065
Antibody Validation:	This antibody replaces catalog number LS-C806. It has been validated for use in the following assays: IHC-P.
Antibody Target:	SMAD, Mothers Against DPP Homolog 6 (SMAD6)
Synonyms:	SMAD6, smad6
Host:	SMAD6 antibody was produced in Rabbit
Antigen Species:	SMAD6 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Synthetic peptide - KLH conjugated
Immunogen:	SMAD6 antibody was raised against synthetic peptide
Antibody Specificity:	Synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG) and amino acids 372-388 (NLEQRSESVRRTRSKIG) of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species.
Antigen Modification:	aa85-99
Predicted Species Reactivity:	Monkey, Mouse, Rat
Purification:	Protein G Column
Presentation:	PBS, 0.05% BSA, 0.05% sodium azide.
Recommended Storage:	Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Antibody Usage Summary:	Immunohistochemistry: LS-B2065 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2065 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (5 - 2 µg/ml) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml