Sheep antibody to c-FOS (2-17): IgG

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

c-Fos; Proto-oncogene protein cFOS; cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7

 

Antibody_Isotype
(Antibody only) : IgG

 

Application_Details :

IHC: Use at an amount of 10 µg/mL with an incubation time of 1-3 days at 4ºC. This antiserum works in paraffin and 4% PFA fixed frozen sections. Penetration is the key to success. Over-fixed tissue is problematic. Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=90

 

Description (Long Description) : 

FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized

 

Immunogen (Antigen) :A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.

 

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