Sheep antibody to c-FOS (2-17): Affinity purified

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Proto-oncogene protein cFOS; c-FOS

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

IH on PFA fixed, frozen tissues. Not yet tested on formalin fixed, paraffin embedded tissue but expected to react. Affinity purified antibody is extremely powerful. A concentration of 1 -5 µg/mL is recommended most uses with short (1-8 hour) incubations. If using enhanced brightfield or amplified detection methods dilutions and long primary antibody incubations dilutions will need to be increased substantially to inhibit background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=155

 

Description (Long Description) : 

FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain

 

Expiry_Date : 

12 months after purchase

 

Format (Form) : 

Lyophilized

 

Immunogen (Antigen) :A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.

 

Kit_Components :

 

MSDS (File name) :