Sheep anti-human Thrombin peroxidase conjugated IgG

Sheep anti-human Thrombin

Peroxidase Conjugated IgG

Description:  Vial containing XXXX ml of of whole IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups.  Total protein is 0.2 mg.

Format:  IgG-HRP conjugate as a clear, slightly red-brown liquid.

Host Animal:  Sheep

Immunogen:  Thrombin prepared from purified human prothrombin, active site blocked with PPACK.

Concentration:  IgG-HRP concentration is XXXX mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14.

Buffer:  A buffered stabilizer solution containing 50% (v/v) glycerol.

Storage:  Store between –10 and –20oC. Product will become viscous but will not freeze. Avoid storage in frost-free freezers.  Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.

Specificity:  Prior to conjugation, this antibody was specific for thrombin or prothrombin as demonstrated by immunoelectrophoresis and ELISA.

Applications:  Suitable as a source of peroxidase labelled antibodies to human thrombin or prothrombin.

Rz Ratio (Reinheitszahl, A403/A280):  XXXX as determined spectrophotometrically