Sheep anti-human Factor VIII purified IgG

Sheep anti-human Factor VIII

Whole IgG from Antiserum

Description:  Vial containing XXXX ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 10 mg.

Format:  Whole IgG, clear liquid.

Host Animal:  Sheep

Immunogen:  Human F.VIII (F.VIII:C) purified from concentrate.

Concentration:  IgG concentration is XXXX mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4.

Buffer:  10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.

Storage:  Store between –10 and –20oC. Product will become viscous but will not freeze. Avoid storage in frost-free freezers.  Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Specificity:  This antibody is specific for F.VIII as demonstrated by immunoelectrophoresis and ELISA.

Applications:  Suitable as a source of antibodies to Factor VIII.

Neutralizing activity:  XXXX Bethesda Units/ml IgG against normal plasma (Kasper CK et al, Thromb Diath Haemorrh 34:869, 1975). One Bethesda unit/ml is defined as the amount of inhibitor that resulted in 50% residual F.VIII activity after 2 hours at 37oC.

Species Cross Reactivity:  Not determined.