Sf9
- 貨號 Cat. No.
- CLC034
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC034) | |
Designations | Sf9 |
| Cell type | Fall armyworn ovary Spodoptera frugiperda |
| Growth | Suspension |
| Seeding density(cells/ml) | 2.0X106 |
Survival rate | 51% |
| Culture medium |
ATCC:TNM-FH medium;To each liter of Grace ’ s Antheraea medium (commercial available,the powder formulation should be prepared according to the manufacturer’s directions before continuing)，add 3.3 /L TC Yeastolate and 3.3 /L Lactalbumin Hydrolysate (both are available from Difco ) Adjust PH to 6.2-6.4 with 1N KOH. Filter sterilize (0.22um) and store at +40C For complete growth medium,add antibiotics (if desired) to each bottle of TNM-FH just prior to use. CCRC:90%Grace”s medium，10% heat-inactivated 10%FBS。
|
| Incubation condition | 270C，No CO2 |
Freezed medium | 93% culture medium+7%DMSO |
Medium renewal | every 2 days |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. |
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.