Serotonin ELISA

pSerotonin ELISA/p
pimg alt=For Research Use Only align=absMiddle src=http://www.alpco.com/img/ruo.gif / For Research Use Only/p
div class=prod_head_infohr /
/div
div class=prod_spec_box
div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
/div
div class=prod_spec_box
div class=prod_specSample Type:/div
div class=prod_spec_infoserum, urine/div
/div
div class=prod_spec_box
div class=prod_specbSample Size:/b/div
div class=prod_spec_info25 micro;l/div
/div
div class=prod_spec_box
div class=prod_specbSize:/b/div
div class=prod_spec_info96 wells/div
/div
div class=prod_spec_box
div class=prod_specbRange:/b/div
div class=prod_spec_info15 - 2500 ng/ml/div
/div
div class=prod_spec_box
div class=prod_specbSensitivity:/b/div
div class=prod_spec_info5 ng/ml/div
/div
div class=prod_spec_box
div class=prod_specbIncubation Time:/b/div
div class=prod_spec_info1 hour(s) 15 min(s)/div
div class=prod_spec_infonbsp;/div
/div
pbSummary:/b/p
pIn the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls, and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations./p
pnbsp;/p