SDF-1beta Antibody

 Antibody Target :SDF-1b

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: E. coli-expressed mouse SDF-1β

Accession#: P40224

Gene ID: 20315

Appearance: Colorless liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) Protein A purified rabbit anti-SDF-1β polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: ms/rt

Application: WB/IP/IHC

Application & Usage: Western blot analysis (0.5-4 µg/ml). Per researcher’s feedback, the antibody can be used in immunoprecipitation (4-8 µg/ml) and immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. Cross-reactivity to SDF-1β of other species has not been determined.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: SDF-1β (stromal cell-derived factor-β), also known as pre-B-cell growth-stimulating factor (PBSF), is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.