SB 203580
- 貨號 Cat. No.
- S-370
- 規格 Size
- 10 x 0.1 mg 1mg 5x1mg
Description
SB 203580
MAPK Homolog - p38 Inhibitor
| Product#: | S-370 |
| Sizes: |
| 10 x 0.1 mg |
| 1 mg |
| 5 x 1 mg |
is highly selective and cell permeable pyridinyl imidazole that inhibits the activity of MAP kinase homologs - p38 (stress-activated protein kinase II/HOG1) and p54 (stress-activated protein kinase I/c-Jun N-terminal kinase) with IC50 values of 50 and 500 nM for p38/SAPK2a and p38/SAPK2b respectively. 1-6 SB203580 also inhibits the phosphorylation and activation of protein kinase B (PKB, also known as Akt) by inhibiting the PKB kinase, phosphoinositide-dependent protein kinase 1 (IC50 3-5 然).7-10
| M.W.: | 377.44 |
| CAS No.: | 152121-47-6 |
| Structure: |
 |
| Purity: | >99% |
| Bioassay: |
| SB203580 inhibits the phosphorylation of Elk1 and Atf2 caused by p38 MAPK. |
 |
3T3-L1 cells were grown to 70% confluence and then serum starved for 3 h. The cells were incubated for 2 h with various concentrations of SB203580 and stimulated with 5mM Anisomycin (#A-520). The cell proteins were resolved by SDS PAGE, probed with anti-phospho-p38 MAPK, anti-p38 MAPK, anti-phospho-Elk1 and anti-phospho-Atf2. The phophorylation of Elk1 and Atf2 was reduced as a function of SB203580 concentrations. |
| References: |
1. | Lee, J.C. et al. (1994) Nature 372, 739. |
2. | Cuenda, A. et al. (1995) FEBS Lett. 364, 229. |
3. | Whitmarsh, A.J. et al. (1997) Mol. Cell. Biol. 17, 2360. |
4. | Clerk, A. et al. (1998) FEBS Lett. 426, 93. |
5. | Hunt, A.E. et al. (1999) J. Biol. Chem. 274, 7591. |
6. | Ward, S.G. et al. (1997) Biochem. Soc. Trans. 2, 304S. |
7. | Ferdinand,V. et al. (2000) J. Biol. Chem. 275, 7395. |
8. | Lali, F.V. et al. (2000) J. Biol. Chem. 275, 7395. |
9. | Martin, M. et al. (2003) Infect. Immun. 71, 2498. |
10. | Darveau, R.P. et al. (2002) Infect. Immun. 70, 1867. |
SB203580