SARS-CoV-2 Nucleocapsid Protein (NP) Human IgM ELISA Kit (1 Plate)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2]

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

This product is intended for use by professional persons only. For research use only. Not for use in clinical & diagnostic procedures. In line with FDA guidelines, test reports must include the following statements:

(1) This test has not been reviewed by the FDA.
(2) Negative results do not rule out SARS-CoV-2 infection, particularly in those who have been in contact with the virus.
(3) Follow-up testing with a molecular diagnostic should be considered to rule out infection in these individuals.
(4) Results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status.
(5) Positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as coronavirus HKU1, NL63, OC43, or 229E.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1735

 

Description (Long Description) : 

The SARS-CoV-2 NP IgM ELISA kit is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection and qualitative measurement of IgM class antibodies against the nucleocapsid protein (NP) of SARS-CoV-2 virus (causing Covid-19) in human blood.

The Biosensis SARS-CoV-2 NP IgM ELISA kit is a two-step incubation immunoassay kit. Recombinant nucleocapsid protein (NP) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-NP antibodies in human serum or plasma specimens. After a 1 hour incubation, anti-NP antibodies are captured by immobilized NP protein while the unbound components are washed away. Afterwards, a detection solution containing HRP-conjugated anti-human IgM is added for another 1 hour incubation, wherein HRP-conjugated anti-human IgM binds to the IgM class antibodies previously bound to NP protein on the plate. After removal of nonspecific bindings, an HRP substrate solution containing 3,3′,5,5′-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H₂SO₄, transforming the blue color to yellow, which is quantified by an absorbance microplate reader at 450 nm. Microplate wells with increased color intensity indicate presence of higher amount of anti-NP antibodies in the tested samples.

This product is intended for use by professional persons only.  For research use only. Not for use in clinical & diagnostic procedures. In line with FDA guidelines, test reports must include the following statements:

(1) This test has not been reviewed by the FDA.
(2) Negative results do not rule out SARS-CoV-2 infection, particularly in those who have been in contact with the virus.
(3) Follow-up testing with a molecular diagnostic should be considered to rule out infection in these individuals.
(4) Results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status.
(5) Positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as coronavirus HKU1, NL63, OC43, or 229E.

 

Expiry_Date : 

The unopened kit is stable for 6 months from date of receipt when stored at 2-8C. Once opened, store reagents at 2-8C for up to 1 month.

 

Format (Form) : 

RUO; Not for diagnostic or clinical purposes; Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.

 

Immunogen (Antigen) :

 

Kit_Components : The ELISA kit box contains 1 x 96-well pre-coated strip plate, blank control sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.

 

MSDS (File name) :