SARS-CoV-2 (COVID-19) S2 protein, His Tag

Source :

SARS-CoV-2 S2 protein, His Tag (S2N-C52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1).

 

Molecule : S2 protein

 

Synonyms : S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2,COVID-19

 

Format : Powder

 

Category : Viral Proteins

 

Accession : QHD43416.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 60.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 20 mM Tris, 150 mM NaCl, pH7.5

 

Description :

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

References :

(1) Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
(2) Benvenuto D, et al. J Med Virol. 2020.
(3) Chang CY, et al. AMB Express. 2020. 10(1):20.