SARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag

Source :

SARS-CoV-2 Papain-like Protease, His Tag (PAE-C5148) is expressed from E.coli cells. It contains AA Glu 746 - Lys 1060 (Accession # YP_009725299.1).

 

Molecule : Papain-like Protease

 

Synonyms : Papain-like Protease,Replicase polyprotein 1a,ORF1a polyprotein,pp1a,1a,PL-PRO,Plpro,COVID-19

 

Format : Liquid

 

Category : Viral Proteins

 

Accession : YP_009725299.1

 

Storage : -70℃

 

Shipping condition : Liquid,-70℃Dry ice

 

Molecular Weight : 37.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 37.6 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 50 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH7.5

 

Description :

New coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Similar to the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), its genome encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus is the same as 3CLpro. This enzyme operates on no less than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential. Therefore, press this The activity of this enzyme will prevent virus imitation.

 

References :

(1) Linlin Zhang, et al. Science. 2020. eabb3405.