SARS-CoV-2 (COVID-19) NSP8 Protein, His Tag

Source :

SARS-CoV-2 NSP8, His Tag (NS8-C5149) is expressed from E.coli cells. It contains AA Ala 1 - Gln 198 (Accession # YP_009725304.1).

 

Molecule : NSP8

 

Synonyms : NSP8,nsp8,Non-structural protein 8,COVID-19

 

Format : Liquid

 

Category : Viral Proteins

 

Accession : YP_009725304.1

 

Storage : -70℃

 

Shipping condition : Liquid,-70℃Dry ice

 

Molecular Weight : 23.9 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 23.9 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 20 mM Tris, 250 mM NaCl, pH8.5

 

Description :

NSP8, forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. To ultimately combat the emerging COVID-19 pandemic, it is desired to develop an effective and safe vaccine against this highly contagious disease caused by the SARS-CoV-2 coronavirus. By investigating the entire proteome of SARS-CoV-2, six proteins, including the S protein and five non-structural proteins (nsp3, 3CL-pro, and nsp8-10) were predicted to be adhesins, which are crucial to the viral adhering and host invasion. The S, nsp3, and nsp8 proteins were also predicted by Vaxign-ML to induce high protective antigenicity.

 

References :

(1) te Velthuis AJ, et al. Nucleic Acids Res. 2012. 40(4):1737-47.
(2) Edison Ong, et al. doi.org. 2020. 000141.