SARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, His Tag

Source :

SARS-CoV-2 NSP7&NSP8, His Tag (NS8-C5125) is expressed from E.coli cells. It contains AA Ser 1 - Gln 83 (NSP7) & Ala 1 - Gln 198 (NSP8) (Accession # YP_009725303.1 (NSP7) & YP_009725304.1 (NSP8)).

 

Molecule : NSP7 & NSP8

 

Synonyms : NSP7 & NSP8,nsp7 & nsp8,COVID-19

 

Format : Liquid

 

Category : Viral Proteins

 

Accession : YP_009725303.1 (NSP7) & YP_009725304.1 (NSP8)

 

Storage : -70℃

 

Shipping condition : Liquid,-70℃Dry ice

 

Molecular Weight : 32.1 kDa

 

Characteristics :

This protein carries a polyhistidine tag.
The protein has a calculated MW of 32.1 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.

 

References :

(1) Krichel B, et al. Biochem J. 2020. 477(5):1009-1019.
(2) Kirchdoerfer RN, et al. Nat Commun. 2019. 10(1):2342.