SARS-CoV-2 (COVID-19) NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag

Source :

SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag (NS0-C51W3) is expressed from E.coli cells. It contains AA Ser 1 - Asn 298 (NSP16) & Ala 1 - Gln 139 (NSP10) (Accession # YP_009725311.1 (NSP16) & YP_009725306.1 (NSP10)).

 

Molecule : NSP16 & NSP10

 

Synonyms : NSP16 & NSP10,nsp16 & nsp10,COVID-19

 

Format : Liquid

 

Category : Viral Proteins

 

Accession : YP_009725311.1 (NSP16) & YP_009725306.1 (NSP10)

 

Storage : -70℃

 

Shipping condition : Liquid,-70℃Dry ice

 

Molecular Weight : 35.3 kDa (NSP16) & 18.3 kDa (NSP10)

 

Characteristics :

SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag is produced by co-expression of NSP16 and NSP10, has a calculated MW of 35.3 kDa (NSP16) and 18.3 kDa (NSP10). Subunit NSP16 is fused with a polyhistidine tag at the N-terminus and subunit

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

NSP10, Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and 2'-O-methyltransferase (NSP16) activities. Therefore plays an essential role in viral mRNAs cap methylation. 2'-O-methyltransferase (NSP16) that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system. Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities.

 

References :

(1) Wang Y, et al. J Virol. 2015. 89(16):8416-27.