SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

Source :

SARS-CoV-2 NSP1, His Tag (NS1-C51H7) is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1).

 

Molecule : NSP1

 

Synonyms : NSP1,nsp1,Leader protein,Host translation inhibitor nsp1,COVID-19

 

Format : Powder

 

Category : Viral Proteins

 

Accession : YP_009725297.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 21.7 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, Arginine, pH7.4

 

Description :

The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.

 

References :

(1) Dong S, et al. J Med Virol. 2020.
(2) Gottipati K, et al. Virology. 2020. 544:31-41.