S5a UIM Peptide agarose
- 貨號 Cat. No.
- AM-110
- 規格 Size
- 0.5 ml
- 品牌 Brand
- Boston Biochem
Description
S5a UIM Peptide agarose
human recombinant
Product Information
0.5 ml
S5a UIM II covalently coupled to agarose at 5 mg/ml resin (2 mM). Resin supplied in 50 mM Hepes pH 7.5, 500 mM NaCl.
Use & Storage
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.
Catalog #: AM-110
S5a UIM Peptide agarose
Product Source:
human recombinant
S5a (Rpn10) is a component of the regulatory complex (19S) of the 26S proteasome. By binding to and recognizing poly-ubiquitinated proteins, it functions as a receptor for proteins destined for proteolytic degradation. S5a has a low affinity for mono-, di- and tri-Ub but binds efficiently to tetrameric ubiquitin and has a preference for longer polymers. The protein recognizes ubiquitin conjugates via two UIM (ubiquitin-interacting motif) domains at located at residues 211-230 (I) and 282-301 (II). Although both UIMs bind to poly-ubiquitin in vitro, UIM II has a 10-fold higher affinity for ubiquitin than UIM I. This affinity resin can be used for the enrichment, isolation and identification of ubiquitinated proteins, proteins that contain ubiquitin-like domains and/or 26S substrates.
Product Information
Quantity:
0.5 ml
Stock:
S5a UIM II covalently coupled to agarose at 5 mg/ml resin (2 mM). Resin supplied in 50 mM Hepes pH 7.5, 500 mM NaCl.
Use & Storage
Use:
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
Storage:
The agarose can be re-used for at least 5-10 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C and 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.