Proteins and Peptides
S100A8 Antibody (LS-B2247)
- 貨號 Cat. No.
- LS-B2247
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2247 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C25355. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Calgranulin A (S100A8) |
| Synonyms: | S100A8, calgranulin a, cfag, cystic fibrosis antigen, leukocyte l1 complex light chain, migration inhibitory factor-related protein 8, mrp-8, s100 calcium-binding protein a8, s100a8 |
| Host: | S100A8 antibody was produced in Mouse |
| Antigen Species: | S100A8 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Modifications: | Fluorescein (FITC) |
| Antigen Type: | Cells |
| Immunogen: | S100A8 antibody was raised against human monocytes |
| Antibody Specificity: | Recognizes human L1 Protein or Calprotectin Molecule. Variable results have been reported for the staining of brain macrophages and microglia. Species cross-reactivity: equine, Cynomolgus monkey, Rhesus monkey, monkey, bovine, baboon, rabbit, canine, feline, porcine, guinea pig and rat. |
| Predicted Species Reactivity: | Bovine, Cat, Dog, Horse, Monkey, Porcine, Rabbit, Rat |
| Purification: | Protein G Column |
| Presentation: | 1% BSA, 0.09% sodium azide. |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2247 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2247 was determined to be 1:50. Flow Cytometry: Neat. Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (1:50), Flow Cytometry (Flo) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.1 mg/ml |


